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Genomics Core Facility

Welcome to the Nova Southeastern University Genomics Core Facility (NSU GCF).  Our goal is to provide NSU investigators, as well as external investigators, access to sophisticated methodologies in genomics including, but not limited to, Illumina next-generation sequencing (NGS) and multiplexed digital profiling of single target molecules using NanoString's nCounter technology.  We ensure high quality data using highly stringent quality control (QC) metrics. 

The critical objectives of the NSU GCF are:

  • To provide investigators with access to high quality, state of the art, sophisticated technology and methodologies for the analysis of research specimens
  • To provide education into the theories and practices of current and emerging genomics technologies
  • To provide excellent comprehensive customer service 

For more information, please email the Genomics Core Facility. Driving directions to CCR may be accessed here.

Upcoming Grant Opportunity 

2018 Illumina RNA-Seq Pilot Grant Program 

NSU’s Translational Research and Economic Development Genomics Core Facility is soliciting applications for pilot projects utilizing the Illumina NextSeq 500 platform for RNA-Seq applications. The primary goal of this pilot grant program is to offer NSU faculty and graduate students (with PI support) a unique opportunity for gene expression research projects at minimal cost as a way to catalyze further next-generation sequencing (NGS) studies. The proposed research must have relevance to basic, translational, and/or clinical science. This opportunity is made available through a generous donation of sample preparation kits and sequencing consumables from Illumina, Inc. The Genomics Core Facility will provide the labor requested for the sample preparation and sequencing free of charge. Please refer to the 2018 Illumina RNA-Seq Pilot Grant Program for full details.

Eligibility Requirements

  • Applications open to NSU faculty and graduate students with PI support 
  • Only one entry per applicant will be accepted.
  • Applicants who are new NGS customers to the NSU Genomics Core Facility will have preference, but any new project to the core is eligible.
  • Applicants must be able to leverage the TruSeq Stranded mRNA kit (library capture of both coding RNA, as well as multiple forms of non-coding RNA that are poly-adenylated).
  • Other reagents and equipment necessary to complete the project are not included in the grant award. Applicants will be required to cover core facility supply and equipment usage charges.

Submission Terms and Conditions

  • The deadline for applications is Wednesday, October 31st 2018.
  • Applications will first be reviewed:
    • For relevance to the impact of basic, translational and clinical research
    • For the potential of publication and application of research grants, and
    • For the appropriate and effective use of the TruSeq Stranded mRNA library prep kit and NextSeq 500 platform
  • Over $4000 in sequencing reagent awards will be granted to two individuals. The Genomics Core Facility will perform the labor required to complete both projects free of charge. Two awardees will be announced on or around Wednesday, November 14th 2018.
  • Sample submission should occur by Friday, November 30th 2018. Minimum sample submission size is 8 and maximum is 24.
  • This is a reagent and labor award only. Awardees will receive 1 sequencing kit (75 cycles high output ) and 50% off reagents for library preparation.  Investigators are responsible for the additional supply and equipment costs incurred to complete the project.
  • Please inquire with Emily Blake in advance of submitting your application for an estimate of additional project costs.

 Application Requirements

  • The length of the Research Abstract is limited to 500 words or less.
  • The Research Proposal Abstract is to include:
    • A brief description of the overall research project
    • Justification for use of the resource requested 
    • Discussion of the impact of the proprosed work on future funding prospects
    • A list of all project collaborators (including a statement of financial support from the PI for graduate student applicants)
  • Apply here!

Upcoming Seminars

 rna-seq_seminar

Join us for a one-hour seminar to learn how Illumina RNA sequencing (RNA-Seq) can help you uncover the answers to drive your research forward. A simple end-to-end sequencing workflow lets you go from sample to results—faster and easier than ever before. During this seminar, Illumina Sequencing Specialist, Matt Angel, will present an overview of next-generation sequencing technology and discuss the basic concepts and key advantages of RNA-Seq. From measuring gene and transcript abundance to uncovering novel features in the coding transcriptome, the possibilities are limitless. See what NGS can do for your research!

Date: Thursday, November 1 2018

Time: 2:00 PM - 3:00 PM

Location: Chancellor's Dining Room (5th floor of Terry Administrative Building/HPD)

Please RSVP!

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