TRED Annual Report FY2021

NSU DIVISION OF RESEARCH AND ECONOMIC DEVELOPMENT NSU DIVISION OF RESEARCH AND ECONOMIC DEVELOPMENT 13 12 NSU’s Core Research Facilities, located in the CCR, promote research excellence, offering five different facilities available to all NSU research programs and their collaborating partners. Operating as fee-for-service facilities, all are available to external researchers as well. For a description of services, equipment, and pricing, or to request a quote, please visit -facilities or email Robin Krueger, Ph.D., scientific director for research facilities at FLOW CYTOMETRY NSU’s Flow Cytometry Core Facility provides researchers with sophisticated methods and instrumentation in flow cytometry, for the fluorescence analysis and preparative sorting of both human and mouse cells to the BSL-2 plus level. The principal assets of the core consist of an analytic instrument (one BD Fortessa X-20 SORP) and one sorting instrument (BD AriaFusion SORP). The core also provides data analysis support and operates workstations with FACSDiva and FlowJo software. IMAGING NSU’s Imaging Core Facility provides users with state-of- the-art imaging instrumentation and microscopy training. The principal assets of the core consist of the following: a confocal microscope (Zeiss LSM 880), an inverted automated imaging system (Life Technologies EVOS), and a highcontent screening platform (CellInsight CX7). NSU CORE FACILITIES GENOMICS AND BIOINFORMATICS NSU’s cutting-edge Genomics Core Facility (GCF) provides access to sophisticated technologies for the study of functional genomics with a focus on rapid run nextgeneration sequencing (NGS) on Illumina’s NextSeq 500 platform and direct multiplexed measurement of gene expression using NanoString’s novel digital color-coded barcode technology on the nCounter analysis system. The GCF specializes in library construction for various sequencing applications including whole genome sequencing, exome sequencing, and transcriptomic profiling. Support services using customized open-source and commercial software for data analysis are offered in conjunction to enhance research project goals. NSU RESEARCH INSTITUTES AND CENTERS To efficiently address key issues facing humanity, NSU has established several research institutes and centers using a multidisciplinary, interprofessional approach. These currently include • B roward County Sea Turtle Conservation Program and Marine Environmental Education Center • C enter For Drug Discovery and Development (CD3) • D eep Pelagic Nekton Dynamics of the Gulf of Mexico (DEEPEND) Consortium • E mil Buehler Research Center for Science, Technology, Engineering, and Mathematics • G uy Harvey Research Institute • I nstitute for Natural and Ocean Sciences Research • I nstitute for Neuro-Immune Medicine • Marilyn Segal Early Childhood Studies Center • N ational Coral Reef Institute • N SU AutoNation Institute for Breast Cancer Research and Care • N SU Cell Therapy Institute • Rumbaugh-Goodwin Institute for Cancer Research • S ave Our Seas Shark Research Center VIVARIUM NSU’s state-of-the-art Vivarium Core Facility is outfitted with Tecniplast Green Line IVC cages. Each room is equipped with temperature, humidity, and light controls. In addition, all holding rooms have VHP ports to maintain an SPF environment. The Vivarium contains two separate holding areas: a barrier suite and a non-barrier suite. NSU’s animal care and use program is accredited by the Association for Assessment and Accreditation of Laboratory Animal Care. CELL THERAPY CORE NSU’s Cell Therapy Core Facility provides expertise in processing of cells from primary tissues, expansion and differentiation of cells, including immune and stem cells, genetic modification of cells by viral vectors, and development of cell-based therapy products, among other services. The main goal is to support research by providing GLP-quality laboratory space, various well-established SOP-driven processes and technical/lab support by experienced staff assistants. We ensure a high quality research environment by providing a HEPA filter particle controlled GLP-quality cell and tissue processing laboratory.