TRED Annual Report FY2018

NSU CENTER FOR COLLABORATIVE RESEARCH 30 The Core Research Facilities located in the CCR promote research excellence, offering five different facilities available to all NSU research programs and their collaborating partners. Operating as fee-for-service facilities, all are available to external researchers, as well. For a description of services, equipment, and pricing, or to request a quote, please visit or email Sara Tavakoli, Ph.D., scientific director for research facilities at . FLOW CYTOMETRY The Flow Cytometry Core Facility provides researchers with sophisticated methods and instrumentation in flow cytometry, for the fluorescence analysis and preparative sorting of both human and mouse cells to the BSL-2 plus level. The principal assets of the core consist of an analytic instrument (one BD Fortessa X-20 SORP) and one sorting instrument (BD AriaFusion SORP). The core also provides data analysis support and operates workstations with FacsDiva and FlowJo software. IMAGING The NSU Imaging Core Facility provides users with state-of- the-art imaging instrumentation and microscopy training. The principal assets of the core consist of the following: a confocal microscope (Zeiss LSM 880), an inverted automated imaging system (Life Technologies EVOS), and a high-content screening platform (CellInsight CX7). CORE FACILITIES GENOMICS AND BIOINFORMATICS NSU’s state-of-the-art Genomics Core Facility (GCF) provides access to sophisticated technologies for the study of functional genomics with a focus on rapid run next-generation sequencing (NGS) on Illumina’s NextSeq 500 platform and direct multiplexed measurement of gene expression using NanoString’s novel digital color-coded barcode technology on the nCounter Analysis System. The GCF specializes in library construction for various sequencing applications including whole genome sequencing, exome sequencing, and transcriptomic profiling. Support services using customized open-source and commercial software for data analysis are offered to enhance research project goals.