TRED Annual Report FY 2017

31 30 NSU CENTER FOR COLLABORATIVE RESEARCH NSU CENTER FOR COLLABORATIVE RESEARCH RESEARCH INSTITUTES AND CENTERS To efficiently address key issues facing humanity, NSU has established several research institutes and centers using a multidisciplinary, interprofessional approach. These currently include • NSU AutoNation Institute for Breast and Solid Tumor Cancer Research • NSU Cell Therapy Institute (a partnership with researchers from Karolinska Institutet in Sweden) • NSU Emil Buehler Research Center for Science, Technology, Engineering, and Mathematics • NSU Institute for Natural and Ocean Sciences Research • NSU Institute for Neuro-Immune Medicine • NSU Rumbaugh-Goodwin Institute for Cancer Research The Core Research Facilities located in the CCR promote research excellence, offering five different facilities available to all NSU research programs and their collaborating partners. Operating as fee-for-service facilities, all are available to external researchers, as well. For a description of services, equipment, and pricing, or to request a quote, please visit facilities or email Sara Tavakoli, Ph.D., scientific director for research facilities at . FLOW CYTOMETRY The Flow Cytometry Core Facility provides researchers with sophisticated methods and instrumentation in flow cytometry for the fluorescence analysis and preparative sorting of both human and mouse cells to the BSL-2 plus level. The principal assets of the core consist of an analytic instrument (one BD Fortessa X-20 SORP) and one sorting instrument (BD AriaFusion SORP). The core also provides data analysis support and operates workstations with FacsDiva and FlowJo software. IMAGING The NSU Imaging Core Facility provides users with state-of-the-art imaging instrumentation and microscopy training. The principal assets of the core consist of the following: a confocal microscope (Zeiss LSM 880 with Airyscan), an inverted automated imaging system (Life Technologies EVOS), and a high-content screening platform (CellInsight CX7). CORE FACILITIES GENOMICS AND BIOINFORMATICS NSU’s state-of-the-art Genomics Core Facility provides access to sophisticated technologies for the study of functional genomics with a focus on rapid run next- generation sequencing (NGS) on Illumina’s NextSeq 500 platform and direct multiplexed measurement of gene expression using NanoString’s novel digital color- coded barcode technology on the nCounter Analysis System. The Genomics Core Facility specializes in library construction for various sequencing applications, including whole genome sequencing, exome sequencing, and transcriptomic profiling. Support services using customized open-source and commercial software for data analysis are offered in conjunction to enhance research project goals. VIVARIUM The state-of-the-art Vivarium Core Facility is outfitted with Tecniplast greenline IVC cages. Each room is equipped with temperature, humidity, and light controls. In addition, all holding rooms have VHP ports to maintain an SPF environment. The Vivarium contains two separate holding areas: a barrier suite and a non-barrier suite. CELL THERAPY The Cell Therapy Core Facility provides expertise in processing of cells from primary tissues, expansion and differentiation of cells including immune and stem cells, genetic modification of cells by viral vectors, and development of cell-based therapy products, among other services. The main aim of the Cell Therapy Core Facility is to support research by providing GLP-quality laboratory space, various well-established SOP-driven manufacturing processes, and technical/lab support by experienced staff assistants. We ensure a high-quality research environment by providing HEPA filter particle controlled antibiotic and a Xeno-free GLP-quality cell and tissue processing laboratory.