TRED Annual Report FY2016

32 NSU CENTER FOR COLLABORATIVE RESEARCH Core Facilities Research Institutes and Centers In order to efficiently address key issues facing humanity, NSU has established several research institutes and centers using a multidisciplinary, interprofessional approach. These currently include • NSU AutoNation Institute for Breast and Solid Tumor Cancer Research • NSU Cell Therapy Institute (a partnership with researchers from Karolinska Institutet in Sweden) • NSU Emil Buehler Research Center for Science, Technology, Engineering, and Mathematics • NSU Institute for Natural and Ocean Sciences Research • NSU Institute for Neuro-Immune Medicine • NSU Rumbaugh-Goodwin Institute for Cancer Research Flow Cytometry Core — The Flow Cytometry Core facility provides users with cutting-edge instrumentation and services, including an analytic instrument (one BD Fortessa X-20 SORP) and a sorting instrument (BD AriaFusion SORP). The core also provides data analysis support and operates workstations with FacsDiva and FlowJo software. Genomics Core — The state-of-the-art Genomics Core facility provides high-quality rapid response services that include whole genome sequencing, exome sequencing, transcriptomics, and gene expression analysis by using high-performance, real-time PCR; Illumina DNA/RNA library preparation; and Illumina Next Generation Sequencing. The high-sensitivity equipment has the ability to profile a low number of cells, small tissues, or small volumes of blood. The 840-square-foot facilities consist of dedicated laboratories for RNA/ DNA extraction, pre- and post-PCR accommodations, an automated sequencing library prep room, high-throughput sequencing, and several instrumentation suites. Cell Therapies Core (CTC) — The CTC has 10 Heracell tissue culture incubators for growing of MSC, one gentle MACS Dissociator (Miltenyi), inverted microscopes, seven biosafety cabinets, two VWR digital water baths, three Sorvall Legend X1R centrifuges, and one Cytospin™ 4 Cytocentrifuge. The CTC is equipped with advanced cell and tissue processing instruments such as AutoMACS Pro, CliniMACS, Wave Bioreactor, GentleMACS Octo, fluorescent microscopy, copper interior split-door CO2 and hypoxic incubators, Akta Pure chromatography, and NanoSight 300. The CTC is capable of design, manufacturing, preclinical evaluation, and optimization of cell therapy products, as well as stem cell differentiation (including embryonic, hematopoietic, and mesenchymal stem cells), in addition to long-term culture and expansion of various cell lineages and cell lines. Common (shared) equipment on the floor will also include one BioSpectrum Imaging Systems UVP LLC for immunoblot imaging and three Cryopro BR-3 LN2 dewars for cell storage. Imaging Core — This facility provides users with state-of-the- art imaging instrumentation and initial training. The principal assets of the core include a confocal microscope (Zeiss LSM 880 with Airyscan), an inverted automated imaging system (Life Technologies EVOS), and a high-content screening platform (CellInsight CX7).