HPD Research Day | February 16, 2018

19 periodontal bone loss via upregulation of OC cell-fusion by upregulation of permissive fusogen CD9, but not DC- STAMP, suggesting that OC-STAMP/CD9 axis induces periodontal bone loss. Resnick Auditorium 11:45 a.m. – 12:15 p.m. Macrophage Migration Inhibitory Factor (MIF) Supports Homing of Osteoclast Precursors to Peripheral Osteolytic Lesions Alexandru Movila, Ph.D., Assistant Professor, College of Dental Medicine, Nova Southeastern University Toshihisa Kawai, DDS, Ph.D., College of Dental Medicine, Nova Southeastern University Background. The present study investigated a possible chemoattractant role of macrophage migration inhibitory factor (MIF), another ligand for C-X-C chemokine receptor type 4 (CXCR4), in the recruitment of circulating OCPs to the bone lytic lesion. Methods. To accomplish this, we used Csf1r-eGFP-knock-in (KI) mice to establish an animal model of polymethylmethacrylate (PMMA) particle-induced calvarial osteolysis. In the circulating Csf1r- eGFP+ cells of healthy Csf1r-eGFP-KI mice, Csf1r+/CD11b+ cells showed a greater degree of RANKL-induced osteoclastogenesis compared to a subset of Csf1r+/RANK+ cells in vitro. Therefore, Csf1r-eGFP+/CD11b+ cells were targeted as functionally relevant OCPs in the present study. Results. Although expression of the two cognate receptors for MIF, CXCR2 and CXCR4, was elevated on Csf1r+/CD11b+ cells, transmigration of OCPs toward recombinant MIF in vitro was facilitated by ligation with CXCR4, but not CXCR2. Meanwhile, the level of PMMA- induced bone resorption in calvaria was markedly greater in wild-type (WT) mice compared to that detected in MIF- knockout (KO) mice. Interestingly, in contrast to the elevated MIF, diminished SDF-1 was detected in a particle- induced bone lytic lesion of WT mice in conjunction with an increased number of infiltrating CXCR4+ OCPs. However, such diminished SDF-1 was not found in the PMMA-injected calvaria of MIF-KO mice. Furthermore, stimulation of osteoblasts with MIF in vitro suppressed their production of SDF-1, suggesting that MIF can downmodulate SDF-1 production in bone tissue. Systemically administered anti-MIF neutralizing monoclonal antibody (mAb) inhibited the homing of CXCR4+ OCPs, as well as bone resorption, in the PMMA-injected calvaria, while increasing locally produced SDF-1. Conclusion. Collectively, these data suggest that locally produced MIF in the inflammatory bone lytic site is engaged in the chemoattraction of circulating CXCR4+ OCPs. Acknowledgements. Grants. This work was supported by NIH grants R03 AG053615 and T32 DE007327. Resnick Auditorium 2:15 – 2:45 p.m. Parents of Non-Special Needs Children and Their Attitude Towards Silver Diamine Fluride as an Alternative Treatment Approach for Carious Lesions Mitchell Miller, DDS, College of Dental Medicine, Nova Southeastern University Judith R. Chin, DDS, MS, Professor, College of Dental Medicine, Nova Southeastern University Romer Ocanto, DDS, Professor, College of Dental Medicine, Nova Southeastern University Maria Levi-Minzi, M.A., College of Arts, Humanities, and Social Sciences, Nova Southeastern University Objective. The goal of this study is to determine the acceptance of using SDF treatment among a non-special needs group of children attending Nova Southeastern Universities (NSU) dental clinics at Joe DiMaggio Children’s Hospital and Kids in Distress in lieu of alternative treatment approaches. Background. Silver Diamine Fluoride is an alternative method to treating carious lesions without the use of a needle and drill to potentially delay or avoid more extensive treatment measures such as oral conscious sedation or general anesthesia. Applied to the caries for 1- 3 minutes, SDF arrests the carious lesion and creates a hard, protective barrier to prevent additional decay [1] [2]. Methods. An 18-question survey was administered to 100 parents of patients being seen at two of NSU’s clinic locations to see if they would select SDF treatment for their child. The clinics utilized for this research were Kid’s in Distress and Joe DiMaggio Children’s Hospital. SDF acceptance was measured by mean scale score on SDF feasibility questionnaire. Scores can range from 0-3, with higher scores indicating higher levels of SDF acceptance. Results. In process, will be completed prior to presentation date. Conclusion. In process, will be completed prior to presentation date. Grant. Grant Number 335612 Research funded by the Health Professions Division grant.