This course will examine the ultrastructure of marine organisms and range in focus from bacteria to fish. Lectures and labs will be conducted to examine scanning and transmission electron microscopy (SEM and TEM) of tissue and cells from many marine groups. Lectures will include discussion of basic theory of electron optics and include elemental analysis (EDS). The preparation of specimens for SEM and TEM including fixation, dehydration, embedding, sectioning, and staining will be discussed in lectures. Additionally, examination of the application of electron microscopy to the field of biomineralization will be discussed. In the labs students will prepare their own research specimens for SEM and/or TEM following the protocols discussed in lecture. Students will conduct presentations and submit a term paper at the end of the semester.
obtain a basic understanding of electron optics
obtain skills required for preparation of specimens for SEM and TEM will be acquire
acquire the knowledge required for operation of the SEM and TEM
use techniques for collection of SEM/TEM images and image processing
understand the relationship between ultrastructure and biomineralization
Recommended - Principles and Techniques of Electron Microscopy: Biological Applications. M. A. Hayat (ed.).2000. 4th ed., CRC Press, Inc., Boca Raton, Florida
Winter 2014 Syllabus
Melissa Bofto and Julio Perez
firstname.lastname@example.org NSU Oceanographic Center
8000 North Ocean Drive
Dania Beach, FL 33004
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The Mission of the Oceanographic Center is to carry out innovative, basic and applied research and to provide high-quality graduate and undergraduate education in a broad range of marine science and related disciplines.