The use of q-PCR has been implemented in microbiology studies to quantify abundance and expression of taxonomic and functional gene markers that pose contamination threats to drinking, recreational, marine, and fresh waters. Its use allows viable results for the indication of microbial presence associated with human pollution that supersedes the abilities of culture based fecal coliform and enterococci studies. The use of PCR chemistries is a more advanced, precise and sensitive method for estimating microbial species in environments. Within PCR chemistries, q-PCR allows for expedient results coupled with greater accuracy to determine if human pollution is contaminating a water source and in what amounts quantitatively.
understand the significance of indicator microorganism to fecal water pollution studies
become familiar with classical culture procedures for determination of fecal coliform and enterococci and the interpretation of their significance
be able to to identify parameters necessary to complete basic stock assessment modeling problems
become familiar with q-PCR procedures to determine the same indicator species as done with culture methods
establish advantages and disadvantages of both techniques and relative specificity to human waste
The Mission of the Oceanographic Center is to carry out innovative, basic and applied research and to provide high-quality graduate and undergraduate education in a broad range of marine science and related disciplines.