This intensive course will
examine the fine structure of marine organisms and range in focus from
bacterial cells to fish tissue. Lectures and labs will be conducted to examine
structure and function of tissue and cells of several marine groups. Light
microscopy in conjunction with molecular methods for study of bacterial cells
such as FISH (Fluorescence In-situ Hybridization) will be discussed.
Additionally, the complementary nature of cell and tissue imaging using light
and electron microscopy will be examined. The lab portion of the course will
follow the protocols discussed in lecture. Fixed and embedded blocks of student
research specimens will be supplied and students will section and stain their
samples for examination in the light microscope. Imaging and image capture
methods including quantification of structural features using ImageJ will be
conducted. Students will prepare their results for presentation and submit a
term paper at the end of the semester.
The students will:
obtain an understanding of the relationship between structure and function of tissue and cells of marine organisms
obtain skills acquired for block sectioning and staining for light microscopy
gain knowledge required for use of the light and epi-floursecent microscope
use of techniques for collection of images and image processing including ImageJ
understand the relationship between histology and ultrastructure
give a formal presentation of histology research results
The Mission of the Oceanographic Center is to carry out innovative, basic and applied research and to provide high-quality graduate and undergraduate education in a broad range of marine science and related disciplines.